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A Representative western blotting images showing the protein expression of p-CaMKII <t>(Thr286)</t> in H1703 and H520 cells incubated with FGF21 for different durations. B Representative Western blotting images showing the protein expression of p-CaMKII (Thr286), CaMKII, p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. C Immunofluorescence staining showing the expression and distribution of p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. D Immunofluorescence staining showing the mitochondrial morphology in H1703 and H520 cells incubated with FGF21 pretreated with KN93. E CCK-8 assay showing the viability of H1703 and H520 cells incubated with FGF21 pretreated with or without KN93. F Representative western blotting images showing the expression of key glycolytic pathway components (GLUT1, LDHA) in DRP1-silenced LUSC cells with or without FGF21 stimulation. GAPDH served as a loading control. The data are shown as the means ± SEMs or SDs; * p < 0.05, ** p < 0.01, *** p < 0.01, **** p < 0.01.
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A Representative western blotting images showing the protein expression of p-CaMKII <t>(Thr286)</t> in H1703 and H520 cells incubated with FGF21 for different durations. B Representative Western blotting images showing the protein expression of p-CaMKII (Thr286), CaMKII, p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. C Immunofluorescence staining showing the expression and distribution of p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. D Immunofluorescence staining showing the mitochondrial morphology in H1703 and H520 cells incubated with FGF21 pretreated with KN93. E CCK-8 assay showing the viability of H1703 and H520 cells incubated with FGF21 pretreated with or without KN93. F Representative western blotting images showing the expression of key glycolytic pathway components (GLUT1, LDHA) in DRP1-silenced LUSC cells with or without FGF21 stimulation. GAPDH served as a loading control. The data are shown as the means ± SEMs or SDs; * p < 0.05, ** p < 0.01, *** p < 0.01, **** p < 0.01.
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A Representative western blotting images showing the protein expression of p-CaMKII <t>(Thr286)</t> in H1703 and H520 cells incubated with FGF21 for different durations. B Representative Western blotting images showing the protein expression of p-CaMKII (Thr286), CaMKII, p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. C Immunofluorescence staining showing the expression and distribution of p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. D Immunofluorescence staining showing the mitochondrial morphology in H1703 and H520 cells incubated with FGF21 pretreated with KN93. E CCK-8 assay showing the viability of H1703 and H520 cells incubated with FGF21 pretreated with or without KN93. F Representative western blotting images showing the expression of key glycolytic pathway components (GLUT1, LDHA) in DRP1-silenced LUSC cells with or without FGF21 stimulation. GAPDH served as a loading control. The data are shown as the means ± SEMs or SDs; * p < 0.05, ** p < 0.01, *** p < 0.01, **** p < 0.01.
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A Representative western blotting images showing the protein expression of p-CaMKII <t>(Thr286)</t> in H1703 and H520 cells incubated with FGF21 for different durations. B Representative Western blotting images showing the protein expression of p-CaMKII (Thr286), CaMKII, p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. C Immunofluorescence staining showing the expression and distribution of p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. D Immunofluorescence staining showing the mitochondrial morphology in H1703 and H520 cells incubated with FGF21 pretreated with KN93. E CCK-8 assay showing the viability of H1703 and H520 cells incubated with FGF21 pretreated with or without KN93. F Representative western blotting images showing the expression of key glycolytic pathway components (GLUT1, LDHA) in DRP1-silenced LUSC cells with or without FGF21 stimulation. GAPDH served as a loading control. The data are shown as the means ± SEMs or SDs; * p < 0.05, ** p < 0.01, *** p < 0.01, **** p < 0.01.
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Image Search Results


A Representative western blotting images showing the protein expression of p-CaMKII (Thr286) in H1703 and H520 cells incubated with FGF21 for different durations. B Representative Western blotting images showing the protein expression of p-CaMKII (Thr286), CaMKII, p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. C Immunofluorescence staining showing the expression and distribution of p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. D Immunofluorescence staining showing the mitochondrial morphology in H1703 and H520 cells incubated with FGF21 pretreated with KN93. E CCK-8 assay showing the viability of H1703 and H520 cells incubated with FGF21 pretreated with or without KN93. F Representative western blotting images showing the expression of key glycolytic pathway components (GLUT1, LDHA) in DRP1-silenced LUSC cells with or without FGF21 stimulation. GAPDH served as a loading control. The data are shown as the means ± SEMs or SDs; * p < 0.05, ** p < 0.01, *** p < 0.01, **** p < 0.01.

Journal: Cell Death & Disease

Article Title: Dual-faced metabolic reprogramming powers proliferation: FGF21 drives lung squamous carcinoma progression via CaMKII-DRP1-mediated mitochondrial fission

doi: 10.1038/s41419-026-09084-3

Figure Lengend Snippet: A Representative western blotting images showing the protein expression of p-CaMKII (Thr286) in H1703 and H520 cells incubated with FGF21 for different durations. B Representative Western blotting images showing the protein expression of p-CaMKII (Thr286), CaMKII, p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. C Immunofluorescence staining showing the expression and distribution of p-DRP1 (Ser616) and DRP1 in H1703 and H520 cells incubated with FGF21 pretreated with KN93. D Immunofluorescence staining showing the mitochondrial morphology in H1703 and H520 cells incubated with FGF21 pretreated with KN93. E CCK-8 assay showing the viability of H1703 and H520 cells incubated with FGF21 pretreated with or without KN93. F Representative western blotting images showing the expression of key glycolytic pathway components (GLUT1, LDHA) in DRP1-silenced LUSC cells with or without FGF21 stimulation. GAPDH served as a loading control. The data are shown as the means ± SEMs or SDs; * p < 0.05, ** p < 0.01, *** p < 0.01, **** p < 0.01.

Article Snippet: Anti-p-CaMKII (Thr286) , 12716 , Rabbit , CST , WB (1:1000).

Techniques: Western Blot, Expressing, Incubation, Immunofluorescence, Staining, Morphology, CCK-8 Assay, Control

List of primary antibodies, secondary antibodies, and nuclear dyes used in this study.

Journal: Cell Death & Disease

Article Title: Dual-faced metabolic reprogramming powers proliferation: FGF21 drives lung squamous carcinoma progression via CaMKII-DRP1-mediated mitochondrial fission

doi: 10.1038/s41419-026-09084-3

Figure Lengend Snippet: List of primary antibodies, secondary antibodies, and nuclear dyes used in this study.

Article Snippet: Anti-p-CaMKII (Thr286) , 12716 , Rabbit , CST , WB (1:1000).

Techniques: